Products Description
Introduction and application of CAS：9028-56-2|3ALPHA-HYDROXYSTEROID DEHYDROGENASE
3α-hydroxysteroid dehydrogenase is a steroid dehydrogenase secreted by Comamonas testosteroni. It consists of 258 amino acid residues and has a theoretical molecular weight of 26.4kD. 3α-hydroxysteroid dehydrogenase can act on a variety of steroid substrates and reversibly catalyze the oxidation and reduction of the hydroxyl/keto group at the 3-position of C19-27 steroids. The isoelectric point of HSD is 4.8, and the optimal pH is 7-9. Hg2+, Ag+, etc. will inhibit enzyme activity. Metal ion chelating agents such as EDTA will not inhibit enzyme activity and are helpful for the stable preservation of enzymes to a certain extent. 3α-hydroxysteroid dehydrogenase is a white yellowish amorphous powder. 3α-hydroxysteroid dehydrogenase is a white yellowish amorphous powder. Bile acid is one of the substrates of 3α-hydroxysteroid dehydrogenase. Clinically 3α-hydroxysteroid dehydrogenase is used to catalyze the dehydrogenation reaction of hydroxysteroids for clinical detection of total bile acids.
Specification of CAS：9028-56-2|3ALPHA-HYDROXYSTEROID DEHYDROGENASE
HSD; 3α-Hydroxysteroid Dehydrogenase from microorganism; 3α-Hydroxysteroid:NAD(P)+ oxidoreductase
Other names: Hydroxysteroid oxidoreductase; hydroxysterol
CAS number: 9028-56-2
Level: BR
Molecular weight: 28KDa (SDS-PAGE)
Vitality: ≥50units/mg protein
Activity definition: One unit will oxidize one micromole androsterone per min at pH 8.9 at 25 °C
Protein：≥25%
Isoelectic point: 7.5
PH stability: 4.5～10.5
PH: 10.5
Temperature: 60℃
Thermal stability: <50℃ (pH7.2, 20min)
Inhibitors: Ag+, Hg2+
Preparation instructions: The enzyme is reconstituted in 30mM potassium phosphate buffer,PH7.2 for activity assay.
Properties (the following information is for reference only): white powder
Purpose: This product is for scientific research only and may not be used for other purposes. (The following uses are for reference only) Catalyze the dehydrogenation reaction of hydroxysteroids.
Storage: -20℃
Package of CAS：9028-56-2|3ALPHA-HYDROXYSTEROID DEHYDROGENASE
1kg/drum
This product is valid for 24 months from the production date when stored in a warm sealed state.
Safety and Transportation
This product should be stored and sealed at room temperature. Customers should carefully read the product treatment, material safety data sheet (MSDS), and labels before use, be familiar with the product's safety information, operating instructions, and environmental impact, and wear appropriate personal protection as required. supplies.
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