CAS：9001-64-3| EC 1.1.1.37

Introduction and application of CAS：9001-64-3| EC 1.1.1.37

Malate dehydrogenase (MDH) exists in all organisms and is one of the key enzymes in biological sugar metabolism. It can catalyze the reversible conversion between malate and oxaloacetate. MDH plays an important role in various physiological activities of cells, including energy metabolism of mitochondria and reactive oxygen metabolism of plants. It has high theoretical research and economic utilization significance. For example, MDH isoenzymes participate in different physiological metabolic pathways of cells: cyMDH is connected to the pyruvate carboxylation branch and is related to non-autotrophic carbon dioxide fixation; mitMDH is a key enzyme in the TCA cycle, providing energy; microbody MDH is related to light Related to respiration or glyoxylate cycle; chloroplast MDH mainly fixes carbon dioxide during photosynthesis. In agricultural production, aluminum toxicity in acidic soil is the main limiting factor for crop growth. Research has found that in order to improve plants' absorption of phosphorus in the soil, plant molecular improvement breeding is carried out through genetic engineering. For example, after overexpressing the MDH gene in alfalfa, the absorption of organic acids by transgenic alfalfa is enhanced, thereby improving the ability to adapt to acidic soil and deal with aluminum. poison tolerance. However, when MDH is highly expressed, the increase in plant organic acid content can increase the osmotic pressure of cells and chelate and remove some ions, thereby improving the plant's tolerance to salt. Recently, it has been proposed to use transgenic technology of halophilic bacteria MDH to improve the salt tolerance of crops. For example, Dunaliella salina D. salina and D. bardwil are the most salt-tolerant eukaryotes discovered so far. By modifying their MDH genes Cloning and sequence analysis can lay the foundation for research on plant salt tolerance.

Specification of CAS：9001-64-3| EC 1.1.1.37

MDH; Malate Dehydrogenase; Malic Dehydrogenase from microorganism
CAS number: 9001-64-3
Molecular weight: 34KDa (SDS-PAGE)
Level: BR
Vitality: ≥200units/mg protein
Vitality definition: One unit will cause the oxidation of one micromole NADH per min at PH7.5 at 30℃
PH: 9.0
PH stability: 4.0～8.0
Temperature: 55℃
Thermal stability:＜60℃(PH 7.5, 1.5min)
Inhibitors: Ag+, Hg2+
Preparation Instructions: The enzyme is reconstituted in 100mM potassium phosphate buffer,PH 7.5 containing 0.2% BSA for activity assay
Properties (the following information is for reference only): white amorphous powder, Isoelectic point: 4.9, when dissolving, use potassium phosphate buffer with a concentration of 50 mmol, the pH is between 6-8, you can add a little 0.2% BSA
Purpose: This product is for scientific research only and may not be used for other purposes. (The following uses are for reference only) Reduce oxaloacetic acid to malic acid or catalyze the dehydrogenation of malic acid to generate oxaloacetic acid
Storage: 2～8℃

Package of CAS：9001-64-3| EC 1.1.1.37

1kg/drum

This product is valid for 24 months from the production date when stored in a warm sealed state.

Safety and Transportation

This product should be stored and sealed at room temperature. Customers should carefully read the product treatment, material safety data sheet (MSDS), and labels before use, be familiar with the product's safety information, operating instructions, and environmental impact, and wear appropriate personal protection as required. supplies.

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