Introduction and application of CAS 207738-08-7 | 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
The enzyme horseradish peroxidase (HRP) has been isolated from the roots of the plant in commercially useful quantities since the nineteen-sixties. Today, HRP-coupled antibodies have become indispensable in immunoassays.
The most popular chromogenic substrate for HRP is Tetramethylbenzidine (TMB), in particular in enzyme-linked Immunosorbent assays (ELISA). HRP converts TMB into a blue color that can be read spectrophotometrically at 650 nm. The HRP reaction can be stopped by adding sulfuric or phosphoric acid. This yields a yellow color that can be read at 450 nm.
Application:
1.Non-carcinogenic alternative to benzidine.
2.A chromophore substrate in enzyme-linked immunosorbent assays.
Synonyms:
(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline,hydrate,dihydrochloride;
3,3 inverted exclamation marka,5,5 inverted exclamation marka-Tetramethylbenzidine dihydrochloride hydrate;
3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate;
3,3‘,5,5‘-Tetramethylbenzidine dihydrochloride hydrate;
TMB dihydrochloride;
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate;
Specification of CAS 207738-08-7 | 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
|
ITEMS |
SPECIFICATION |
|
CAS |
207738-08-7 |
|
Molecular Formula |
C16H22Cl2N2 |
|
Molecular Weight |
313.26 |
|
EINECS |
688-511-0 |
|
Melting point |
>300°C |
|
solubility |
Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml). |
Package and transportation of CAS 207738-08-7 | 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Package:0.1g,1g,5g,25g,100g,500g,1kg
Transportation: by courier/ by air/ by sea
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