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Introduction of CAS 39450-01-6 | Proteinase K
Proteinase K= Protein (long chain of amino acid) + ase (suffix for enzyme) + K (keratin)
Proteinase K is a highly active protease of the subtilisin class, used for the general degradation of proteins in biological samples. , has the advantages of high specific activity, high yield and wider temperature/pH activity range.
Role of proteinase K digestion in DNA extraction
The proteinase K digests mainly the hydrophobic amino acids ( both aromatic as well as aliphatic). The cofactor, calcium ion provides stability to the enzyme, however, it cannot increase the activity of the reaction.
Highest proteinase K activity is reported at the temperature 60ºC ranging from 37ºC to 70ºC. The temperature vs enzymatic activity graph is shown below.
Application of CAS 39450-01-6 | Proteinase K
Used in genetic diagnostic kits, genomic DNA extraction kits, and RNA extraction kits to remove nucleases and other protein contamination;
Extraction of non-protein biological products such as nucleic acid and polysaccharide drugs in the biopharmaceutical industry, such as the preparation of DNA vaccines and heparin; Used in daily industrial fields such as skin health care, removal of aging cuticle cells, skin tanning, preparation of hydrolyzed protein seasoning, cosmetics and feed processing.
[Enzyme activity] ≥30U/mg freeze-dried powder
[Optimum pH range] 7.5-11.5
[Microbiological limit] ≤5 cfu/g
[Optimum temperature range] The recommended temperature range is 37-70℃, with the highest activity at 70℃
[Inactivation method] Add 5mM PMSF to the reaction system to inactivate it.
[Inhibitors] Diisopropylfluorophosphate (DIFP), phenylmethylsulfonyl fluoride (PMSF)
Specification of CAS 39450-01-6 | Proteinase K
|
Items |
Specification |
|
Molecular Weight |
29.7KDa |
|
Appearance |
Lyophilized Powder |
|
Storage conditions |
Store below 4°C |
|
Purity |
Pass , 99% |
|
Enzyme activity |
≥30U/mg dry weight |
|
DNA&RNA Contamination Assay |
None detected |
|
DNase Contamination Assay |
None detected |
|
RNase Contamination Assay |
None detected |
Product Categories Proteinase K
|
Product name
|
Appearance | Enzyme activity | Packaging specifications |
| Proteinase K freeze-dried powder | White or off-white powder | ≥30U/mg | 20mg/bottle, 1g/bottle, 10g/bottle, etc. |
| Proteinase K sterile freeze-dried powder | White or off-white powder | ≥30U/mg | 100mg/bottle, 100g/bottle, etc. |
| Proteinase K liquid (20mg/ml) | Colorless transparent solution | ≥900U/ml | 1ml/bottle, 10ml/bottle, 1L/bottle, etc. |
| Proteinase K liquid (40mg/ml) | Colorless transparent solution | ≥1800U/ml | 1ml/bottle, 10ml/bottle, 1L/bottle, etc. |
| Industrial Grade Proteinase K | Light yellow freeze-dried powder | ≥150U/g | 10g/bottle, 20g/bottle, 50g/bottle, etc. |
20mg/bottle,100mg/bottle, 1g/bottle, 10g/bottle, are packaged according to customer needs
Store refrigerated below 4°C
Solution form Proteinase K
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