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CAS: 9025-65-4 | MUNG BEAN NUCLEASE Benzonase Nuclease

CAS: 9025-65-4 | MUNG BEAN NUCLEASE Benzonase Nuclease

Purity: 40KU 50KU 100KU
Package:1g 25g 100g 1000g
Worldwide Delivery
Made in China

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Product Introduction

Introduction and application of CAS: 9025-65-4 | MUNG BEAN NUCLEASE Benzonase Nuclease

 

Broad-spectrum nuclease Binuclease is a non-specific endonuclease derived from Serratia marcescens (also known as Spiritbacter) that has been modified through protein engineering technology. It does not contain bacterial endotoxins expressed by prokaryotes. Functionally, it can cleave various forms of DNA and RNA, whether single-stranded, double-stranded, linear, circular or superhelical, cutting the phosphodiester bond to produce 5'-phosphate nucleoside acid or 5'-phosphate oligonucleotide. Broad-spectrum nucleases have no specific requirements for nucleic acid base sequences and can cut between any nucleotides in the chain, and can be used to remove nucleic acids in biological products. In the vaccine industry, protein and polysaccharide pharmaceutical industry, it is widely used to remove host nucleic acid residues, which can cause nucleic acid contamination of vaccines and protein products to reach picogram (pg) levels. Broad-spectrum nucleases can also be used as the enzyme preparation of choice for deviscifying cultured cell supernatants and cell lysates.

 

Specification of CAS: 9025-65-4 | MUNG BEAN NUCLEASE Benzonase Nuclease

 

ITEMS

SPECIFICATION

CAS

9025-65-4
E.C 3.1.30.2
Source species 144.21
Molecular weight 27.9KD
Specific activity value >1000KU/mg

Purity

>90%(SDS-PAGE)
Enzyme activity value Solid enzyme activity value>20kU/mg freeze-dried powder; liquid enzyme activity value>250U/uL
endotoxin 0.25EU/KU
Optimum pH pH6-10
Optimum temperature 37°C
Cofactor 1-10mM Mg2+
dilution buffer 20 mM Tris-HCI (pH 8.0),2 mM MgCl,20 mM NaCl
storage buffer 20 mM Tris-HCI (pH 8.0) ,2 mM MgC,20 mM NaCl,50%glycerin
Storage temperature -20 save



Package and transportation of CAS: 9025-65-4 | MUNG BEAN NUCLEASE Benzonase Nuclease

 

Package:10g, 100g, 1000g 5kg

Transportation: by courier/ by air/ by sea

 

product-400-400

 

 

product-750-758

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